MAA-peptide mimotopes are recognized by CLL69C rAb.

(A) Amino acid compositions of MAA-mimotopes P1 and P2 as recently reported [20]. (B) Chemiluminescent ELISA for the binding of increasing concentrations CLL69C rAb to peptide mimotopes P1 and P2, MAA-BSA, or an irrelevant control peptide (pep-ctrl). Data are expressed as RLU/100 msec, and are from three independent experiments (mean ± SD), each value determined in triplicate. (C) Competition immunoassay demonstrates specificity of the CLL69C rAb to mimotope peptide P1. A fixed and limiting concentration of CLL69C Ab was incubated in the absence or presence of increasing concentrations of indicated competitors, and extent of binding to P1-coated plates was determined and expressed as B/B0 from triplicate determinations (mean ± SD) as explained in legend to Fig. 1B.