Lytic DNA replication of 293T-BAC36 and 293T-BACΔ6 cells.

293T cells harboring BAC36, BACΔ6 and BACΔ6 infected with lentiviruses expressing ORF6 were collected at different time points postinduction (0, 48 and 72 h), and total intracellular DNA were extracted. Intracellular viral DNAs were measured by a real-time PCR with primers directed to the ORF73 gene. The viral genome copies were normalized to 20,000 copies of GAPDH.