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Lung histopathological changes after hemorrhagic shock and resuscitation (HSR).

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posted on 07.05.2013, 02:25 by Junko Kosaka, Hiroshi Morimatsu, Toru Takahashi, Hiroko Shimizu, Susumu Kawanishi, Emiko Omori, Yasumasa Endo, Naofumi Tamaki, Manabu Morita, Kiyoshi Morita

Lungs from the HSR group rats treated with or without biliverdin (BV) administration were excised 12 h after resuscitation and subjected to histological analysis. (A) Representative images from five independent experiments (hematoxylin–eosin staining, original magnification ×200, scale bar  = 100 µm). (B) The severity of histopathological changes in the lungs was graded for congestion, edema, inflammation, and hemorrhage. A total of 10 areas of lung parenchyma from each rat were graded as 0 (no findings or normal), 1 (mild), 2 (moderate), or 3 (severe) for each of the four parameters. (C) The sum of histopathological scores for the four parameters were calculated (n = 5 per group). Data are presented as means ± standard deviation and were statistically evaluated using analysis of variance followed by Tukey–Kramer honestly significant difference test. *p<0.01 vs. vehicle/sham; p<0.01 vs. BV/sham; #p<0.01 vs. vehicle/HSR. Vehicle/sham, vehicle-administered animals subjected to sham surgery; BV/sham, BV-administered animals subjected to sham surgery; vehicle/HSR, vehicle-administered animals subjected to HSR; BV/HSR, BV-administered animals subjected to HSR.