Figure_6.tif (135.77 kB)
Loxosceles and Sicarius ornatus venom SMases D incorporate into human erythrocytes and cause loss of glycophorin C expression.
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posted on 2013-08-22, 02:14 authored by Priscila Hess Lopes, Rogério Bertani, Rute M. Gonçalves-de-Andrade, Roberto H. Nagahama, Carmen W. van den Berg, Denise V. TambourgiErythrocytes were treated with venoms from Sicarius ornatus or Loxosceles or with VBS2+ buffer (control) and analyzed for the expression GPC by flow cytometry [A]. The ability of the toxins to insert into the erythrocyte surface was analyzed using a monospecific polyclonal rabbit serum against Loxosceles intermedia SMases D [B]. Results are representative for three different experiments and expressed as median of fluorescence of duplicates ± SD. The variation among experiments was around 10%. (*) Significant differences (P<0.05) from control; (#) Significant differences from L. laeta venom (P<0.05); (•) Significant difference between female and male Sicarius venoms (P<0.05).