Localization of the plasma membrane and tonoplast markers in control and vamp721vamp722 mutant seedlings.

(A) GFP-Lti6a localization in control root. Note (enlargement at right) that GFP-Lti6a remained extensively colocalized with FM4-64 at the plasma membrane. Bars  =  10 µm. (B) GFP-Lti6a localization in vamp721vamp722 roots. Note (enlargement at right) that GFP-Lti6a abnormally accumulated in the cytoplasm. Bars  =  10 µm. (C) PIP2A-GFP localization in control roots. Note (enlargement at right) that PIP2A-GFP remained extensively colocalized with FM4-64 at the plasma membrane. Bars  =  10 µm. (D) PIP2A-GFP localization in vamp721vamp722 roots. Note (enlargement at right) that PIP2A-GFP accumulated inside aberrant intracellular compartments. Bars  =  10 µm. (E) and (F) TIP1;1-GFP showed similar tonoplast labeling patterns in cells of control (E) and vamp721vamp722 (F) root tips. Bars  =  10 µm. (G) and (H) TIP1;1-GFP-labeled tonoplast and small vacuoles in cells of control (G) and vamp721vamp722 (H) hypocotyls. Red signals represent chloroplast autofluorescence. Bars  =  10 µm.