Localization of E-cadherin and γ-catenin is altered in flotillin-2 depleted MCF10A cells.

(A) The expression level of various cell-cell contact proteins is not altered in MCF10A cells depleted of flotillin-1 (sh-F1-A/B) or flotillin-2 (sh-F2-A/B). Flotillins were stably knocked down in MCF10A cells by means of lentiviruses carrying respective shRNAs. Expression of indicated proteins was studied in cell lysates by menas of Western blot. (B) MCF10A cells depleted of flotillin-1 (sh-F1-A) or flotillin-2 (sh-F2-A) were grown for 3 days on coverslips, fixed and stained with antibodies against flotillin-2, E-cadherin and γ-catenin. Scale bar: 20 µm. (C) and (D) Quantification of relative cell border distribution of E-cadherin and γ-catenin in MCF10A cells depleted of flotillin-1 (sh-F1-A/B) or flotillin-2 (sh-F2-A/B). Data points represent the mean ± SD of three independent experiments in which 20 cell-cell borders were measured. One-way ANOVA with Bonferroni’s multiple comparison test. *, p<0.05; **, p<0.01; ***, p<0.001.