Liposomes were not significantly lysed by exposure to community exoproducts for four hours.
This data is compiled from three independent experiments each with three biological replicates and error bars represent SD. Data was analyzed by one-way ANOVA and a Dunnett’s post-test was used to compare all groups to heat-killed biofilm supernatant ‘HK sup’ (first bar). Statistical symbols: NS = not significant; *** = p < 0.001. Abbreviations: Mix = supernatant from mixed culture; Triton = Triton X-100 treatment; PlcH = treatment with 1 ng/ul purified PlcHR (CITE); Pa Sup = supernatant from Pseudomonas aeruginosa PAO1 as a postitive lipolytic control.