Light microscopy and fluorescence microscopy images of non-differentiated and differentiated THP-1 cells.

Non-differentiated THP-1 cells (A) display a round shape and a nonadherent pattern while differentiated THP-1 cells (B) display a dendritic shape and an adherent pattern at light microscopy (×400), black scale bar : 10 µm. Fluorescence microscopy images (×200) showing CD11c expression by non-differentiated THP-1 cells (C) and differentiated THP-1 cells (D). Nuclei are stained in blue (DAPI) and CD11c is stained in red phycoerythrin (PE), white scale bar : 20 µm. A significantly increased CD11c expression was observed on differentiated THP-1 cells.