Levels of megalin and STAT3 and pSTAT3 protein expression in Lou/C and Wistar rats.
A) Representative Western blot of megalin in choroid plexus. B) Representative Western blots of STAT3 and pSTAT3 in hypothalamus. C) Schematic representation of leptin receptor activation in POMC neurons of the ARC nucleus of the hypothalamus. D) Representative Western blots of FoxO1 in hypothalamus. All Western blots were revealed using horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence detection. The quantifications were performed using the ChemiDocTM XRS and the Quantity OneTM software. Results obtained 30 minutes after a leptin challenge of 2 mg/kg i.p., were normalized with actin protein expression. Values are mean + SEM of 4 to 5 animals per group. *p<0.05 compared to Wistar rats using the Student’s t test.
