Knockdown of Grb2 during EB formation.
figure
posted on 2013-07-18, 02:47 authored by Yang Liu, Qidong Liu, Wenwen Jia, Jie Chen, Jianmin Wang, Dan Ye, Xudong Guo, Wen Chen, Guoping Li, Guiying Wang, Anmei Deng, Jiuhong Kang(A) Western blotting analysis demonstrates that shGrb2 knocks down Grb2 protein levels in ES cells. (B) Relative levels of layer markers are varied in shGrb2-treated EBs. (C) The in situ expression of layer markers in day 10-differentiated EBs. Red, layer markers; blue, nuclear DNA staining. The scale bar represents 100 µm. (D) Erk activity decreases in response to Grb2 knockdown. All data are expressed as the means ± SD. Statistical significance was assessed by the two-tailed Student’s t test. ***, p < 0.001; **, p < 0.01; *, p < 0.05.
