Knockdown of CREB with siRNA attenuates PGE₂-induced IL-6 protein expression in orbital fibroblasts.

A: siRNA specific to CREB (CREB si) or scrambled siRNA (Control si) was transfected into 80% confluent cultures. Representative Western blot analysis demonstrates the impact of the knockdown of CREB protein. B: Media were subjected to IL-6 ELISA. Data are expressed as the mean ± SD of three independent determinations These results were representative of those in two other orbital fibroblast strains.