Fig_3.tif (2.82 MB)
Knockdown of AKT3 increases the expression of S100A4 protein.
figure
posted on 2016-01-18, 15:40 authored by Astrid Grottke, Florian Ewald, Tobias Lange, Dominik Nörz, Christiane Herzberger, Johanna Bach, Nicole Grabinski, Lareen Gräser, Frank Höppner, Björn Nashan, Udo Schumacher, Manfred Jücker(A) AKT signaling pathway activity after downregulation of AKT isoforms was analyzed by Western blot with antibodies directed against the indicated proteins. HSC70 was used as loading control. (B) Relative mRNA levels of S100A4 were measured by qPCR (Bars: SD. *, p < 0,05. **, p < 0,01). (C) Expression of S100A4 in MDA-MB-231 control and AKT isoform knockdown cells was analyzed by Western blot, HSC70 was used as loading control. (D) S100A4 expression was quantified from Western blot analysis of triplicates and data were normalized to corresponding HSC70 controls (Bars: SD. *, p < 0,05).
