Kinetics of formation of amyloid fibrils followed by ThT fluorescence at 37°C.

A: Kinetic curves of amyloid fibrillation of N47A Spc-SH3 in the presence of 100 mM NaCl at different initial protein concentrations as indicated. B: Double logarithmic plot of the initial rates of amyloid fibrillation of N47A Spc-SH3 versus the initial protein concentration in presence of different NaCl concentrations: 50 mM (blue); 100 mM (red) and 200 mM (green). The initial rates of the double mutant K43A-N47A Spc-SH3 in the presence of 100 mM NaCl are shown in magenta. Symbols correspond to the experimental rates and continuous lines represent the best fits according to equations 4 to 8 of the model as described in the text.