Kinetic and dose effects of TNF and WA/Cel combination on XIAP and IAP1/2 in cells.

(A) Kinetic effects of TNF-α plus WA/Cel on XIAP and IAP1/2. Cells were treated with WA (2.5 µM)/Cel (1 µM) with or without TNF-α (10 ng/ml) for the indicated times, followed by Western blotting for XIAP and IAP1/2. (B) Dose effect of TNF-α plus WA/Cel on XIAP and IAP1/2. Cells were treated with the indicated doses of WA/Cel with or without TNF-α (10 ng/ml) for 24 hours, followed by measurement of XIAP, IAP1/2 by Western blotting. (C) Effect of TNF-α plus WA (upper)/Cel (lower) on XIAP mRNA. Cells were treated with WA (1, 2 µM)/Cel (0.5, 1 µM) with or without TNF-α (10 ng/ml) for 12 hours, followed by measurement of XIAP mRNA by RT-qPCR. (D) Effect of TNF-α plus WA (upper)/Cel (lower) on cIAP1/2 mRNA. Cells were treated with WA (1, 2 µM)/Cel (0.5, 1 µM) with or without TNF-α (10 ng/ml) for 12 hours, followed by measurement of cIAP1/2 mRNA by RT-qPCR. Data are shown as mean ± SD of three experiments. (*P<0.05, as compared with the untreated control. #P<0.05, WA/Cel treated versus WA/Cel+TNF-α).