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K02288 induces dorsalization of zebrafish embryos.

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posted on 2013-04-30, 01:19 authored by Caroline E. Sanvitale, Georgina Kerr, Apirat Chaikuad, Marie-Christine Ramel, Agustin H. Mohedas, Sabine Reichert, You Wang, James T. Triffitt, Gregory D. Cuny, Paul B. Yu, Caroline S. Hill, Alex N. Bullock

(A) Brightfield photographs of 26 hours old Tg(BRE:mRFP) transgenic embryos treated with DMSO or varying doses of K02288 from the 8- to 16-cell stage. Severity of the dorsalization correlated with the dose of K02288. Very strong dorsalized phenotypes were observed with 8–10 µM K02288. (B) The phenotypes of the embryos shown in A were classified according to Kishimoto et al. [49]. (C) Western blot for mRFP in extracts prepared from Tg(BRE:mRFP) embryos treated in a parallel experiment. Loss of mRFP protein was evident at 8–10 µM K02288. As a control, the effects of dorsomorphin (DM) and LDN-193189 (LDN) on mRFP expression are also shown. Protein loading control is shown with the MCM6 blot.

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