Intracellular ROS levels assayed with DCFH-DA fluorescent probe in porcine granulosa cells treated with ZEA.
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posted on 2015-06-01, 05:09 authored by Xunsi Qin, Mingjun Cao, Fangnong Lai, Fan Yang, Wei Ge, Xifeng Zhang, Shunfeng Cheng, Xiaofeng Sun, Guoqing Qin, Wei Shen, Lan LiA (A’), Control (untreated); B (B’), 15 μM ZEA; C (C’), 30 μM ZEA; D (D’), 60 μM ZEA. Intracellular ROS was measured by flow cytometry (A-D) and fluorescent imaging (A’-D’) using DCFH-DA probe. For better clarity, a guidance line (red) is drawn through histograms for comparison. E, Average intensity from DCFH-DA. The results are presented as mean ± SD. Asterisk (*) indicates significant difference (P<0.05).
