Inhibition of transcription or translation induces mitotic slippage in nocodazole treated HEK293 cells.

Characterization of HEK293 cells: control cells, nocodazole (noc)-treated cells (arrested in mitosis), noc-cells incubated with cycloheximide (CHX; 35.5 µM) or actinomycin D (ActD; 8 µM) for 6 h. A) Phase-contrast microscopy; magnification: 200×. B) Mitotic index of noc-treated cells with or without CHX or ActD for 6 h. Data presented as mean ± S.D. from three independent experiments; ** p<0.01 and *** p<0.005 noc-CHX or noc-ActD versus noc.