Induction of UGT activity by epirubicin.
figure
posted on 2012-10-22, 01:44 authored by Ryan W. Dellinger, Harry H. Matundan, Amelia S. Ahmed, Priscilla H. Duong, Frank L. Meyskens Jr.Glucuronidation activity was examined by the UGT-Glo Assay using 50 µg of homogenate per reaction in three melanoma cell lines; (A) WM3211 (B) SKmel28 and (C) A375 without treatment and compared to treatment with epirubicin (EPI) at the indicated concentrations for 24 hrs. (−) indicates a negative control in which no homogenate was present. (+) indicates use of human liver microsomes (known to have high concentrations of almost all UGT family members) as a positive control.
