Individual trajectory and mean-squared displacement (MSD) of an AChR particle.

The upper row shows the displacement of the same particle at the different time points indicated in the graph. The MSD (t) of the fluorescent-labeled particle was calculated for the initial 15 time lag intervals, Δt, for trajectories longer than a critical number (15) of frames. In the example shown, the time-series was acquired at 20°C in a control CHO-K1/A5 cell labeled with Alexa⁴⁸⁸-α-BTX for 1 h at 4°C. The 2-dimensional MSD (t) for each particlés trajectory (Δr(Δt))², was calculated for every time interval [28]–[30].