Increased phosphorylation of p38-MAPK in mDCs and monocytes from HIV-infected patients following stimulation with L. plantarum.

(A) Representative flow cytometric gating strategy detecting phosphorylation of p38 in mDCs following stimulation with L. plantarum WCFS1. Myeloid dendritic cells were gated based on forward and side scatter followed by lineage-negative (CD3-CD14-CD16-CD19-CD20-CD56-), and were positive for both HLA-DR and CD11c. (B) Frequencies of mDCs phosphorylated p38 following stimulation with L. plantarum WCFS1 as determined by phosflow (HIV− n = 7; HIV+ n = 14). (C) Representative flow cytometric gating strategy detecting phosphorylation of p38 in monocytes following stimulation with L. plantarum WCFS1. Monocytes were gated based on forward and side scatter and were positive for the lineage marker, HLA-DR, and CD11c. (D) Frequencies of monocytes phosphorylated p38 following stimulation with L. plantarum WCFS1 as determined by phosflow (HIV− n = 7; HIV+ n = 14). Each dot represents an individual subject and bars indicate median. P values determined using Mann Whitney U test.