Increased levels of acetycholinesterase (AChE) mRNA variants in cirrhotic liver.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Relative mRNA levels of the liver transcripts for AChE (T, R and H; labelled AChE-T, AChE-R and AChE-H in the figure) were analyzed by QRT-PCR in non-diseased (NL; ▪; n = 5) and cirrhotic liver cases (CL; □; n = 9). The selected primers were: AChE-T transcript: Fw: 5′-CTTCCTCCCCAAATTGCTC3-′, Rev: 5′-TCCTGCTTGCTGTAGTGGTC-3′; AChE-R transcript: Fw: 5′-CTTCCTCCCCAAATTGCTC-3′, Rev: 5′-GGGGAGAAGAGAGGGGTTAC-3′; AChE-H transcript: Fw: 5′-CAATGAGCCCCGAGACC-3′, Rev: 5′-GAGCCTCCGAGGCGGTG-3′; GAPDH: Fw: 5′-AGCCACATCGCTCAGACAC-3′, Rev: 5′-GCCCAATACGACCAAATCC-3′. Values were calculated using relative standard curves and normalized to GAPDH. Specificity of the PCR products was confirmed by dissociation curve analysis. *p<0.05.