In vivo selective targeting of astrocytes by SindMu-ZsGreen/GLAST lentiviruses.

A. Broad-field images of brain sections following transduction with sindMu-ZsGreen/GLAST IgG lentivirus. The figure shows defined areas in the brain and cell types that were transduced and expressed viral-mediated ZsGreen. panel a - ZsGreen lentiviral expression; panel b - GFAP staining for astrocytes; panel c - NeuN staining for neurons; panel d - merged image for ZsGreen expression in neurons (NeuN) and astrocytes (GFAP). Indicated scale bar, 200µm (zoom x10).

B. Higher magnification of sindMu-ZsGreen/GLAST IgG transduction - engineered lentiviruses were injected into the hippocampus and thalamus of mice, and 14 days post injection brain slices were generated and stained with the appropriate antibodies. panel a - ZsGreen lentiviral expression; panel b - GFAP staining for astrocytes; panel c - NeuN staining for neurons; panel d – merged image for ZsGreen, GFAP and NeuN expression. Arrows present cells were GFAP and ZsGreen are co expressed. Indicated scale bar, 50µm (zoom x40).

C. Single cell imaging of the above transductions with sindMu-ZsGreen/GLAST IgG lentiviruses. Analysis shows preferential targeting of astrocytes in the CNS of mice by SindMu-ZsGreen/GLAST-1 IgG. panel a - ZsGreen lentiviral expression; panel b - GFAP staining for astrocytes; panel c – merged image for ZsGreen and GFAP expression at the level on a single cell. Indicated scale bar - 20µm (zoom x100).