In vivo effect of NIR-PIT for MDAMB468 tumor (one shot NIR-PIT).

(A) NIR-PIT regimen. Fluorescence images were obtained at each time point as indicated. (B) In vivo fluorescence real-time imaging of tumor bearing mice in response to NIR-PIT. The tumor treated by NIR-PIT showed decreasing IR700 fluorescence after NIR-PIT. (C) Tumor growth was significantly inhibited in the NIR-PIT treatment groups with cet-IR700 (n = 10–11, **p < 0.01 vs control and light only group, Bonferroni’s test with ANOVA). (D) Significantly prolonged survival was observed in the APC i.v. only group and the NIR-PIT treatment group with cet-IR700 (n = 10–11, *p < 0.05 vs control, ^#p < 0.05 vs i.v. group, **p < 0.001 vs control and light only group, by Log-rank test).