In vitro assembled baculovirus comet tails.
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posted on 2014-01-14, 02:47 authored by Jan Mueller, Julia Pfanzelter, Christoph Winkler, Akihiro Narita, Christophe Le Clainche, Maria Nemethova, Marie-France Carlier, Yuichiro Maeda, Matthew D. Welch, Taro Ohkawa, Christian Schmeiser, Guenter P. Resch, J. Victor SmallImages of viruses and actin tails obtained in in vitro assays after negative staining and conventional transmission EM. (A) Budded baculovirus in the infected cell supernatant. (B) De-enveloped virus obtained after detergent treatment of the budded virus. (C–E) Actin comet tails formed on baculovirus in vitro in the motility cocktail after the incubation times indicated. (F, G) Effect of varying gelsolin concentration on the length of the comet tail filaments. Bars (A, B, F), 100 nm; (C, D, E, G), 500 nm.
