Immunohistochemical staining of AGEs in the hippocampus and cortex.

Mice were injected (i.p.) with Rib at dose of 2 g/kg, or Glc at dose of 2 g/kg, or 0.9% saline (controls) for 30 days. AGEs in the mouse brain were detected by immunohistochemistry using anti-AGEs monoclonal antibody. The areas outlined with dashed lines are magnified in the lower panels (bar  =  50 µm).