Immunohistochemical staining and semi-quantitative analysis of VEGF and Col I.

(A, C) Immunohistochemical assays of the expression of VEGF (A) and Col I (C). (B, D) Bar graphs represent the expression density of VEGF and Col I as unit area of bone marrow or unit area of bone trabeculae. Transplantation of MSCs reversed the decline of VEGF and Col I expression in the marrow, but decreased the accumulation of Col I in the trabeculae. The most significant recovery was seen in the ONFH+MSC+HGF group. Quantification was based on at least 10 fields per section. *P<0.05 vs. normal group.