Immunofluorescence with anti-histone H3S10P antibody.

(A, B) Examples of binucleated and mononucleated cells from a 15 day-old mouse. In this young mouse the mononucleated cells show different status of chromatin modification as detected by an anti phospho-histone H3 antibody (green). Small H3S10P foci or a diffuse staining (white arrows) are indicative of non replicative cells, while larger H3S10P foci at pericentric chromatin (red arrows) suggest that cells are proceeding to the G2/M phase. (C-F) Cells from a 5 month-old mouse. (B,D,F) Binucleated cells from young (B) and old (D,E) mice with small H3S10P foci (B,D) and with dot-like structures (F). The staining for both nuclei is comparable.