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Immunofluorescence staining of DD-POR, Golgi apparatus, and ER.

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posted on 2014-11-21, 03:05 authored by Ramona Dettwiler, Andrea L. Schmitz, Philippe Plattet, Jana Zielinski, Meike Mevissen

V79-CYP3A94/DD-POR cells (POR) and V79-CYP3A94 were incubated with or without Shield-1 (1 µM), 1 µM) for 6 hours. The anti-DD antibody (red) was used as marker for POR, calnexin and gigantin were used as specific marker for ER (A) and Golgi apparatus (B) (green), respectively. Nuclei were stained with Hoechst 33342 (bottom row). Scale bar 10 µm for all images.

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