Immunofluorescence showing NF-κB p65, E2A, FOXO1 and FOXP1 localization in the MCF-7 cell line.
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posted on 2013-02-20, 17:58 authored by Zhengshan Chen, Yanna Xiao, Junjun Zhang, Jing Li, Yuxuan Liu, Yingying Zhao, Changchun Ma, Jin Luo, Yamei Qiu, Guowei Huang, Christine Korteweg, Jiang GuA, normal mouse IgG was used instead of the primary antibody. B, the primary antibody was mouse anti-E2A. C, the primary antibody was mouse anti-NF-κB p65. A to C, the secondary antibody was goat anti-mouse IgG-FITC. D, normal rabbit IgG was used instead of the primary antibody. E, the primary antibody was rabbit anti-FOXO1. F, the primary antibody was rabbit anti-FOXP1. D to F, the secondary antibody was goat anti-rabbit IgG-TRITC. Similar results were obtained for the A549, PC3 and MDA-MB-231 cell lines (data not shown).
