Immunofluorescence assay using Vpr-GFP, HA-Vpr and sdAbs Vpr1A and Vpr2A.
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posted on 2014-12-01, 03:05 authored by Julie Matz, Cécile Hérate, Jérôme Bouchet, Nelson Dusetti, Odile Gayet, Daniel Baty, Serge Benichou, Patrick ChamesImmunofluorescence was performed by cotransfecting Hela cells with a plasmid coding for Vpr-GFP (B) or HA-Vpr (C) and increasing amount of a plasmid coding for a sdAb. Vpr-GFP, HA-Vpr and sdAbs were tracked as described in Fig. 3. The coefficient of Pearson (coefficient of co-localization) was calculated for each cell cotransfected with Vpr-GFP and a sdAb.
