Imaging cardiovascular geometries with cryo-EFIC.

a) The cryo-EFIC machine and its components. b) Sample EFIC image slice of a newborn mouse, demonstrating that there were contrasts between different tissues to distinguish the organs. Int - intestines; c) Demonstration of segmentation: rendered image of the blood volumes (gray) of a newborn mouse, with the aorta and its head and neck arterial branches set apart in red. d) Segmented great arterial geometries of wildtype fetal mice over stages E14.5 to E18.5 compared to that of the newborn mouse. IVC – inferior vena cavae; Li – liver; Lu – lungs, RA - right ventricle; RV – right ventricle; Sp – spine; AAo: ascending aorta; DAo: descending aorta; PT: pulmonary trunk; Duc: ductus arteriosus; Innom: innominate; LCC: left common carotid; LSC: left subclavian.