Identification of trachoma conserved antigens by western blotting using chlamydial antigen specific antibodies.
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posted on 2013-05-30, 08:53 authored by Laszlo Kari, Lauren E. Bakios, Morgan M. Goheen, Leah N. Bess, Heather S. Watkins, Timothy R. Southern, Lihua Song, William M. Whitmire, Norma Olivares-Zavaleta, Harlan D. CaldwellIP was performed using monkey RML134 serum and chlamydial proteins prepared from non-denaturing detergent lysed non-radiolabeled A2497 infected McCoy cells. The IP proteins we identified by Western blot using a panel of rabbit monospecific antibodies generated against PmpD and mouse monoclonal antibodies specific to CPAF and Pgp3. (A) The higher MW polypeptides were identified as full length PmpD (155 kDa) and its proteolytically processed 82 kDa translocator and 73 kDa passenger domains [10] (B) Two lower MW polypeptides reacted with antibodies specific to CPAF and Pgp3.
