Figure_5.tif (2.53 MB)
Identification of proteins forming SDS-insoluble aggregates in cells expressing 103Q-GFP.
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posted on 2014-12-30, 03:06 authored by Anton A. Nizhnikov, Alexander I. Alexandrov, Tatyana A. Ryzhova, Olga V. Mitkevich, Alexander A. Dergalev, Michael D. Ter-Avanesyan, Alexey P. Galkin2D-DIGE image of proteins of aggregates isolated using SDS (A). Spots corresponding to proteins from cells expressing 103Q-GFP and 25Q-GFP (control) are red and green, respectively. SDS-PAGE image of proteins from the same DRAFs that were insoluble in UTC buffer (B). Mr – protein molecular mass markers. Proteins identified by mass-spectrometry are indicated. Identification data are presented in Table 5 and S5 Table.