Figure_2.tif (757.96 kB)
IMAC purification of His6-HwTx-IV(acid).
figure
posted on 2013-12-06, 03:09 authored by Isabelle Sermadiras, Jefferson Revell, John E. Linley, Alan Sandercock, Peter RavnA) Slow gradient elution IMAC. Lane M, Seeblue pre-stained molecular weight marker (Invitrogen); Lanes 1 to 26, fractions collected during imidazole gradient elution. Elution of monomers starts at 60mM imidazole. At 105mM imidazole, only monomers elute. B) Capture IMAC with step elution at 105mM Imidazole. Lane M, Seeblue pre-stained molecular weight marker (Invitrogen); Lanes 1 to 12, fractions collected during the 105mM imidazole elution; Lanes 13 and 14, fractions collected during the second elution step at 500mM imidazole.
