ID8-bearing mice develop iron deficiency anemia.

C57BL/6 mice were injected intraperitoneally with 1×10⁶ ID8 cells and euthanized after 18.5 weeks. Compared to controls, ID8-bearing mice: (A) are more anemic; (B) have elevated ZPP levels; (C,D) have lower liver and spleen iron levels. N = 10–16 mice per treatment and control group. *P<0.05, **P<0.001; P by Mann-Whitney rank-sum test (A,B) or student t-test (C,D). Bars and error bars are median ± median ±75^th/25^th percentile (A,B) or mean ± SD (C,D).