Hypoxic regulation of NF-kB nuclear expression in LNCaP, DU145 and PC3 cells.

Cell were exposed to 1% O₂ from 0.5 up to 24 h or left untreated. After the times indicated, cells were processed and the nuclear content of p50 and p65 was detected by immunoblot analysis. β-actin was used as a loading control. A representative experiment for each gene in all the studied cell lines is shown. Mean densitometry of NF-kB p50 and p65 relative to β-actin ± SE is also evidenced and expressed as arbitrary units. * Hypoxic cells vs normoxic control cells.