Human α-syn expression in the striatum and SN following intranigral rAAV2/5-α-syn injection.

Representative samples of human α-syn immunoreactivity in rat striatum (A, C) and substantia nigra (B, D) following intranigral injection with rAAV2/5-α-syn. A-B. Transduction following injection of 1.0 x 10¹³ vg/ml titer rAAV2/5-α-syn. (A) α-syn immunoreactivity is observed throughout the striatum ipsilateral to injected nigra. Insert shows striatal α-syn positive neurites and protein aggregates (20X). (B) α-syn immunoreactivity observed in the injected SN. C-D. α-syn expression following injection of 1.0 x 10¹⁴ vg/ml titer rAAV2/5-α-syn. α-syn immunoreactivity in the ST (C) and SN (D) following vector injection. Insert shows α-syn positive nigral neurons at 60X. E-L. α-syn co-expression and aggregation within SNpc THir neurons. E-H. Lower magnification image of α-syn immunoreactive aggregation within THir neurons and surrounding neurites. I-L. THir neurons with (white arrow heads) and without (yellow arrow heads) α-syn accumulation at 168x.