Hsp90α/β and FN localisation in MDA-MB-231, Hs578T and FG cells.

Fixed MDA-MB-231, Hs578T and FG cells stained using mouse anti-human FN and goat anti-human Hsp90α/β followed by donkey anti-mouse DyLight® 488 and donkey anti-goat DyLight® 650 fluorescent secondary antibodies, respectively. Nuclei were stained with Hoechst 33342 (1 µg.ml⁻¹). Images were captured using a Zeiss LSM 510 Meta laser scanning confocal microscope and analyzed using Zen, blue edition, Zeiss, Germany. White arrows show regions of interest for co-localisation, gray arrows show regions where co-localisation is not prevalent. PDM values and a Colour Scatter Plot were generated using Image J. Positive and negative PDM values are indicated in orange and blue respectively. Scale bars are equivalent to 10 µm. The data shown are representative of triplicate experiments.