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Homozygous knockout mutant line mrs2-10.

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posted on 2010-08-23, 01:12 authored by Anne M. Visscher, Anna-Lisa Paul, Matias Kirst, Charles L. Guy, Andrew C. Schuerger, Robert J. Ferl

(a) A homozygous T-DNA insertion mutant for AtMRS2-10 (At1g80900) was identified by PCR based on the SALK_100361.41.30.x line. (b) RNA extracted from mrs2-10 and Col-0 leaves was tested for the presence of MRS2-10 mRNA with gene-specific primers. Alpha-(α)-Tubulin (TUB) was used as a constitutive control.

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