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Homodimerization of SULT4A1.

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posted on 2014-07-02, 03:04 authored by Neelima P. Sidharthan, Neville J. Butcher, Deanne J. Mitchell, Rodney F. Minchin

(A) Conservation of the dimerization motif in various human SULTs. The consensus sequence is shown below. (B) Immunoprecipitaton assay for dimerization of SULT4A1 protein. IMR-32 cells were co-transfected with HA-tagged SULT4A1 and either FLAG-tagged wild-type SULT4A1 (WT), mutant SULT4A1 (TV) or empty vector (EV). Isolated cytosolic proteins were immunoprecipitated with anti-HA antibody and detected with anti-FLAG antibody. Western blots of protein lysates prior to immunoprecipitation (Input) demonstrate expression. Western blots using anti-HA antibody (lower panel) demonstrates immunoprecipitation.

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