Heteronuclear NOE experiment on P143T EvpP.

(A) Overlay spectra from heteronuclear NOE experiments showing cross-peaks from an experiment without proton saturation (red) and an experiment with proton saturation (black). The label next to each peak corresponds to sequential assignment of the residue. (B) A plot showing the relative ratio of peak intensities with proton saturation against those without proton saturation for all residues that can be assigned. Residue with a low value for the ratio is located at a disordered region. Boundaries of the three identified ordered regions are boxed by dashed lines and labeled Box 1, 2 and 3.