HaCaT, HEKn (passage 3) and HEK-CaY (passage 10) cells were cultured with human foreskin fibroblasts in a 3D human skin equivalent model for 28 days in vitro.

Gross morphology was compared to normal human skin by H&E (A) to assess ability to for an organised stratified epidermis. Expression of stratification markers K14 (B), K10 (C) and Inv (D) was also assessed by immunohistochemistry. Dashed lines indicate the location of the basement membrane. Scale bar is equivalent to 20μm. All images are taken at 20x magnification and are representative of three experimental repeats.