HSFY expression in different tissues.

HSFY was analyzed in a variety of tissues including testis (T), lung (Lu), heart (H), liver (Li), kidney (K) and ovary (O) using real time PCR and two different HSFY primer sets to amplify the gene as well as the reference gene, GAPDH. The results show that both HSFYRNA (HSFYRNAF/HSFYRNAR) and HSFY8 (HSFY8F/HSFY8R) primer sets amplify HSFY only in the testis and no other tissue. All tissues show expression of GAPDH. M) 100 bp ladder (Invitrogen Canada Inc.).