Figure_7.tif (107.29 kB)
HSFY expression in different tissues.
figure
posted on 2013-02-20, 20:08 authored by Christine K. Hamilton, Tamas Revay, Robin Domander, Laura A. Favetta, W. Allan KingHSFY was analyzed in a variety of tissues including testis (T), lung (Lu), heart (H), liver (Li), kidney (K) and ovary (O) using real time PCR and two different HSFY primer sets to amplify the gene as well as the reference gene, GAPDH. The results show that both HSFYRNA (HSFYRNAF/HSFYRNAR) and HSFY8 (HSFY8F/HSFY8R) primer sets amplify HSFY only in the testis and no other tissue. All tissues show expression of GAPDH. M) 100 bp ladder (Invitrogen Canada Inc.).