Figure_6.tif (811.95 kB)
HD5 is present in infected urine samples.
figure
posted on 2013-02-20, 09:03 authored by John David Spencer, David S. Hains, Edith Porter, Charles L. Bevins, Julianne DiRosario, Brian Becknell, Huanyu Wang, Andrew L. SchwadererHD5 levels in sterile urine samples (n = 15) and urine samples infected with uropathogenic E.coli (n = 15). Cationic peptides from non-infected urine (NL) and infected urine (IN) were subjected to SDS PAGE followed by Western immunoblot analysis. Each lane contained the equivalent of 300 µg of urinary cationic protein. (A) Silver stained PAGE gels with 150 ng ProHD5 as standard to confirm equal protein loading into each lane. (B) Western blot analysis with 200 ng ProHD5 as standard.
