Growth kinetics of rpoB, inhA and ftsZ KD strains.
figure
posted on 2015-08-06, 03:21 authored by Sudha Ravishankar, Anisha Ambady, Haripriya Ramu, Naina Vinay Mudugal, Ragadeepthi Tunduguru, Anand Anbarasu, Umender K. Sharma, Vasan K. Sambandamurthy, Sudha RamaiahKD cultures grown with minimum IPTG required were harvested, washed and used for preparing inoculum of cultures for growth kinetic studies. A master culture of each KD strain with ~106 CFU/ml was prepared, split into many aliquots where each aliquot received a different IPTG concentration. They were grown at 37°C. The effect on the growth was monitored by measuring the survivors periodically by plating them on 7H11 plates supplemented with IPTG. rpoB (A), inhA (B) and ftsZ (C). The graphs are representative results obtained from 3 independent experiments.
