Granule cell precursor proliferation was decreased in the cerebellums of Rere^om/eyes3 embryos at E18.5.

A-F. Proliferation assays were performed on cerebellar vermis sections prepared from the cerebellums of wild-type and Rere^om/eyes3 embryos and mice at E18.5 (A–B), P0 (C–D) and P3 (E–F) using an anti-Phospho Histone H3 antibody. A-B. The number of mitotic granule cell precursors in Rere^om/eyes3 embryos appeared to be lower than that of their wild-type littermates at E18.5. C-F. Mitotic activity of GCPs was comparable between Rere^om/eyes3 embryo and wild-type littermate at P0 and P3. G. Phospho-Histone H3-positive cells in the entire external granule cell layer (EGL) were counted and normalized by the area of the EGL (n≥3 with twenty slides containing three sections for each genotype). The number of Phospho-Histone H3-positive GCPs per µm² was reduced in Rere^om/eyes3 embryos at E18.5 when compared to wild-type littermates (* = p<0.03) but was indistinguishable between Rere^om/eyes3 embryos and wild-type littermates at P0 and P3. ABL, anterobasal lobe; ADL, anterodorsal lobe. Scale bar  = 200 µm.