Glucose intolerance and impaired glucose-induced insulin secretion in S2814D mice.

(A, B) Glucose tolerance tests were conducted by injecting glucose (2 g/kg body weight) in WT and S2814D mice after a period of fasting for 6 hours. Quantifaction revealed glucose intolerance in S2814D mice as evidenced by higher blood glucose levels (A) and the lack of an insulin response (B). (C) Insulin tolerance tests were conducted by injecting insulin (0.75 U/kg body weight) after 6 h of fasting. (D) Acute phase insulin secretion in response to glucose (3 g/kg body weight) after 16 hours of fasting revealed a blunted response in S2814D mice. Data are represented as average ± SEM. N = 4−14 mice per group. **P<0.01, *P<0.05 vs. WT.