Genistein treatment corrects behavioural abnormalities seen in MPSIIIB mice.
(A) 8 month old MPSIIIB and WT, male and female mice with and without long-term genistein treatment were monitored in the open field test of locomotor and exploratory activity for 60 minutes. The arena was divided into 12 squares and the frequency of entry (and total duration- not shown) to the central 4 squares measured to determine responses to danger and thigmotaxis. (B) The average speed in the central area and (C) side squares was also measured, as was (D) total distance travelled, to give an indication of abnormal locomotor activity. (E) Rapid exploratory behaviour was measured by frequency and (F) duration of speed more than 90 mm/s. (G) The number of times and (H) the duration of time spent immobile was also measured as speed under 0.05 mm/s. (I) Latency to cross or fall from a hanging bar is a measure of locomotor activity with some element of cognitive function due to a training period and was tested at 10 months of age. Significant gender*genotype effects were seen in centre and side speed, distance travelled, frequency of speed over 90 mm/s, immobility frequency and duration reflecting greater pathological effect in female MPSIIIB mice. For all graphs genders were pooled, thus n = 12–14 mice per group, error bars represent SEM, p values are for Tukey's multiple comparisons test.