Genetic complementation rescued protoperithecial development in all three MAP kinase mutants.

(A) Young protoperithecium of a rescued Δos-2 transformant (NCAL020) enwrapped by enveloping hyphae. Granulated ECM depositions as seen on Δos-2 hyphae (Figure 7A) could no longer be observed in the rescued Δos-2 transformants, which showed smooth hyphal surfaces evenly covered in ECM (compare to wild type in Figure 7E). Scale bar, 20 µm; in inset 5 µm. (B) Mature protoperithecium of the rescued Δos-2 transformant. Scale bar, 20 µm. (C) VHF (arrowheads) undergone in the rescued Δmak-1 transformant (NCAL010). Scale bar, 20 µm. The inset shows an ascogonial coil of this strain from which a straight hypha emerges which resembles a trichogyne initial (arrowhead). Scale bar, 5 µm. (D) Mature protoperithecium of the rescued Δmak-1 transformant. Scale bar, 20 µm. (E) CAT-mediated cell fusion (arrowheads) in a rescued Δmak-2 transformant (NCAL043). Scale bar, 20 µm. (F) Mature protoperithecium of a rescued Δmak-2 transformant. Scale bar, 20 µm.