Generation of Lrp1b null alleles.
figure
posted on 2013-02-21, 01:55 authored by Martin F. Dietrich, Louise van der Weyden, Haydn M. Prosser, Allan Bradley, Joachim Herz, David J. Adams(A) Duplication of N-terminal exons 6-8 to generate the Lrp1btm1wtsi allele and Southern blot hybridization after EcoRV digestion of embryonic stem cell genomic DNA to verify targeting of the allele. (B) Duplication of C-terminal exon 69 to generate the Lrp1btm2wtsi allele and Southern blot hybridization after BamHI digestion of embryonic stem cell genomic DNA to verify targeting of the allele. A, AflIII; B, BamHI; EV, EcoRV; S, SwaI.
